The purpose of this research is to know and analyze the Determination of Leadership, Organizational Culture, and Work Motivation on Work Performance of the University of Batam. The research method used is survey method with causal technique because of determination of some variable to Lecturer Performance. The population is Lecturer at the University of Batam and the number 114 Lecturers.  The sample size was 114 lecturers with the sampling technique used by the census. This study uses questionnaire instruments to measure four variables: Leadership, Organizational Culture, and Lecturer Motivation, and test tools to measure variables using Performance as an Intervention variable. These devices have been piloted before being used to collect data, and the results of each instrument are valid and reliable. The data analysis technique uses one lane analysis, which is used to test the significance of each path coefficient. The result of this study is that Leadership determination significantly on Performance, Organizational Culture determination not significant to Performance, Motivation determination considerably to Performance, Leadership, Organizational Culture and Motivation determination substantially to Performance. The conclusion of this research is Leadership, Culture Organization, Motivation as an essential variable that determines the performance of lecturers. Therefore, the above variables should get attention from the leaders of the University of Batam to improve the performance of lecturers.